It is the inventor of a bespoke minicircle technology for AAV and CAR-T cell production, and the only manufacturer that can …
Produce high-purity plasmid and minicircle DNA across a range of quality grades
Generate difficult-to-produce and unstable plasmids and minicircles (e.g. PolyA120, ITRAAV)
Produce up to 10 g of plasmid material per run with low chromosomal DNA impurities
Provide plasmid and minicircle DNA using antibiotic-free fermentation processes
Guarantee a fixed quantity of material, rather than simply selling a production run
Provide AAV 2-Plasmid-System for cotransfection
Request adviceScaling up: We offer DNA products of the highest purity in greater quantities than ever.
We provide different quality levels for research and (pre-) clinical studies to ensure optimal adaption to your application. Our High Quality grade* plasmids and minicircles are particularly suitable for GMP-compliant production of viral vectors and RNA, for example.
*HQ: High Quality Grade is produced in accordance with EMA guideline CHMP/BWP/2458/03 as the highest non-GMP quality standard **On request
ApplicationsKit alternative for basic research
ApplicationsOptimized, highly efficient DNA for basic research, pre-clinical and toxicology studies
ApplicationsOptimized, highly efficient for basic research, pre-clinical and toxicology studies, enzyme-free
ApplicationsDNA starting material for early clinical phase GMP production of e.g. AAV, CAR-T cells or mRNA vaccines
ApplicationsDNA of highest quality as starting material for clinical phases and market supply or DNA for direct human application
Show entire tablePlasmidFactory produces plasmids and minicircles in modern laboratories according to the requirements of customers worldwide, applying the highest standards and continuously optimized methodologies developed by our own sophisticated R&D department.
We indeed have a very fruitful and productive scientific collaboration with PlasmidFactory for more than 10 years now.
Prof. Dr. Michael Hudecek, Universitätsklinikum Würzburg/2023
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Manufacturing of customized plasmid DNA Manufacturing of customized minicircle DNA In-Stock products for AAV production In-Stock products (reporter-plasmids/-minicircle) cooperation opportunities Other topicI would like a specific quote for the following product
Customized DNA AAV plasmids und minicircles reporter gene plasmids and minicircles pEPito plasmids Molecular Size Markers CGE Service Storage Serviceprovide required
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Get in touch PublicationsPublications about Minicircle DNA
The following literature on the topics plasmids, DNA manufacturing, gene therapy, and genetic vaccination can be requested at PlasmidFactory (contact@plasmidfactory.com). Books can only be purchased through the bookstore.
M. Izco, M. Schleef, M. Schmeer, E. Carlos, G. Verona and L. Alvarez-Erviti, Targeted Extracellular Vesicle Gene Therapy for Modulating Alpha-Synuclein Expression in Gut and Spinal Cord, Pharmaceutics 2023, 15, 1230 (2023)
R. Shankar, N. Schäffer, C. Krüsemann, M. Schmeer, M. Schleef, In-process controls for plasmid isoform characterization, Abstract ESGCT 29th Annual Congress in collaboration with BSGCT Edinburgh, UK October 11–14, 2022 Published Online:14 Dec 2022
D. Lock , R. Monjezi, C. Brandes , S. Bates, S. Lennartz, K. Teppert , L. Gehrke, R. Karasakalidou-Seidt, T. Lukic, M. Schmeer, M. Schleef, N. Werchau, M. Eyrich , M. Assenmacher, A. Kaiser, S. Prommersberger, T. Schaser, M. Hudecek, Automated, scaled, transposon-based production of CAR T cells, open access, Journal for Immuno Therapy of Cancer (2022)
S. Prommersberger, R. Monjezi, R. Shankar, M. Schmeer, M. Hudecek, Z. Ivics et al., Minicircles for CAR T Cell Production by Sleeping Beauty Transposition: A Technological Overview, Gene Therapy Of Cancer – Methods and Protocols (2022)
M. Schmeer, M. Schleef, R. Shankar, D. Kobelt, W. Walther, Capillary Gel Electrophoresis (CGE) for Quality Control of Plasmid DNA in Gene Therapy: Quality Control of 20 Years Stored GMP-Grade Plasmid DNA, Gene Therapy Of Cancer – Methods and Protocols (2022)
R. Shankar, N. Schäffer, M. Schmeer, J.M. Risse, K. Friehs & M. Schleef, Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification. Microb Cell Fact 20, 171 (2021).
M. Izco, J. Blesa, M. Schleef, M. Schmeer et al., Systemic Exosomal Delivery of shRNA Minicircles Prevents Parkinsonian Pathology, Molecular Therapy Vol.27 No 12 Dec., The American Society of Gene and Cell Therapy (2019)
T. Buchholz, M. Schleef (2018), Classification and Regulation of Advanced Therapy Medicinal Products, International Biopharmaceutical, Industry, Volume 1 Issue 3, 44-45, Winter 2018
T. Buchholz, C.J. Christel and H. Besir (2018), Customised Viral Engineering - Proactive Progress, European Biopharmaceutical Review, April, 24-31.
M. Holstein, C. Mesa-Nuñez, C. Miskey et al. (2018), Efficient Non-viral Gene Delivery into Human Hematopoietic Stem Cells by Minicircle Sleeping Beauty Transposon Vectors, Molecular Therapy Vol. 26 No 4, 1137-1153, April 2018
T. Buchholz, M. Schmeer and M. Schleef (2017), Size Matters, European Biopharmaceutical Review, October, 68-70.
M. Schmeer, T. Buchholz and M. Schleef (2017), Plasmid DNA Manufacturing for Indirect and Direct Clinical Applications, Human Gene Therapy, 28(10):856-861.
R. Shankar, M. Schmeer and M. Schleef (2017), Minicircles: next-generation gene vectors, Cell Gene Therapy Insights 017;3(2), 285-300.
Wiebke Garrels, Thirumala R. Talluri, Maren Ziegler, Ilka Most, Diego O. Forcato, Marco Schmeer, Martin Schleef, Zoltán Ivics and Wilfried A. Kues (2016), Cytoplasmic injection of murine zygotes with Sleeping Beauty transposon plasmids and minicircles results in the efficient generation of germline transgenic mice, www.biotechnology-journal.com
M. Schnödt, M. Schmeer, B. Kracher, C. Krüsemann, L. E. Espinosa, A. Grünert, T. Fuchsluger, A. Rischmüller, M. Schleef and H. Büning (2016), DNA Minicircle Technology Improves Purity of Adeno-associated Viral Vector Preparations, Molecular Therapy—Nucleic Acids, 5, e355.
R. Monjezi, C. Miskey, T. Gogishvili, M. Schleef, M. Schmeer, H. Einsele, Z. Ivics and M. Hudecek (2016), Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors, Leukemia, 1-9.
I. Freudenau, P. Lutter, R. Baier, M.Schleef, H. Bednarz, A. R. Lara and K. Niehaus (2015), ColE1-plasmid production in Escherichia coli: mathematical simulation and experimental validation, Frontiers in Bioengineering and Biotechnology, 10.3389/fbioe.2015.00127.
H. Akita, D. Kurihara, M.Schmeer, M. Schleef and H. Harashima (2015), Effect of the Compaction and the Size of DNA on the Nuclear Transfer Efficiency after Microinjection in Synchronized Cells, Pharmaceutics, 10.3390/pharmaceutics7020064.
M. Schmeer, M. Schleef (2014), Pharmaceutical Grade Large-Scale Plasmid DNA Manufacturing Process, in: M. Rinaldi et al. (eds), Methods in Molecular Biology, DNA Vaccines, vol. 1143, 219-240.
D. Kolbelt, M. Schleef, M. Schmeer, J. Aumann, P.M. Schlag,W. Walther (2013), Performance of High Quality Minicircle DNA for In Vitro and In Vivo Gene Transfer, Mol. Biotechnol., 53 (1), 80-89.
S. Chabot, J. Orio, M. Schmeer, M. Schleef, M. Golzio, J. Teissié (2013), Minicircle DNA Electrotransfer for efficient Tissue targeted Gene Delivery, Gene Therapy 20 (1), 62-68.
M. Viefhues, S. Wegener, A. Rischmüller, M. Schleef and D. Anselmetti (2013), Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines, Lab Chip, 13 3111-3118.
M. Schleef (2013), Non Viral DNA Vectors, In: D. Scherman (ed.), Advanced Textbook on Gene Transfer, Gene Therapy and Genetic Pharmacology – Principles, Delivery and Pharmacology and Biomedical Application of Nucleotide-Based Therapies, Imperial College Press London, 183-205.
M. Schleef (2013), Production and Purification of Plasmid Vectors, In: D. Scherman (ed.), Advanced Textbook on Gene Transfer, Gene Therapy and Genetic Pharmacology – Principles, Delivery and Pharmacology and Biomedical Application of Nucleotide-Based Therapies, Imperial College Press London, 517-525.
W. Walther, M. Schmeer, D. Kobelt, R. Baier, A. Harder, V. Walhorn, D. Anselmetti, J. Aumann, I. Fichtner, M. Schleef, A seven-Year Storage Report of Good Manufacturing Practice-Grade Naked Plasmid DNA: Stability, Topology, and In Vitro/In Vivo Functional Analysis, HGT Clin. Dev. 24, 147-153.
M. Schleef (ed.) (2013), Minicircle and Miniplasmid DNA Vectors, The Future of Non-Viral and Viral Gene Transfer, Wiley-VCH Weinheim, ISBN 978-3-527-32456-9.
M. Schleef, M. Blaesen, M. Schmeer, R. Baier, C. Marie, G. Gickson, D. Scherman (2010), Production of non-viral DNA Vectors, Current Gene Therapy, 10, 487-507. Press London, 183-205.
M. Schmeer (2009), Electroporative Gene Transfer, in: W. Walther and U. S. Stein (eds), Methods in Molecular Biology, Gene Therapy of Cancer, vol. 542, 157-165. Press London, 183-205.
M. Schleef, M. Blaesen (2009), Production of Plasmid DNA as a Pharmaceutical, in: W. Walther and U. S. Stein (eds), Methods in Molecular Biology, Gene Therapy of Cancer, vol. 542, 471-495.
C. Maucksch, A. Bohla, F. Hoffmann, M. Schleef, M. K. Aneja, M. Elfinger, D. Hartl, C. Rudolph (2009), Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian cells, J Gene Med 11: 444-453.
M. Schleef, R. Baier, W. Walther, M.L. Michel, and M. Schmeer (2006), Long-Term Stability Study and Topology Analysis of Plasmid DNA By Capillary Gel Electrophoresis, BioProcess International, 38-40.
M. Schleef (ed.) (2005), DNA-Pharmaceuticals, Wiley-VCH Weinheim, ISBN 3-527-31187-4.
R. Shankar, M. Schmeer, M. Schleef, Producing Plasmid DNA Template for Clinical Grade RNA Vaccine Manufacture. In: Kramps, T. (eds) RNA Vaccines. Methods in Molecular Biology, vol 2786. Humana, New York, NY. (2024)
